What is a recombinant nucleosome?
A recombinant nucleosome is a laboratory-assembled complex of DNA wrapped around histone proteins, mimicking the natural structure found in chromatin. By combining defined histones and DNA in a controlled way, recombinant nucleosomes provide a uniform, reproducible, and physiologically relevant tool for studying chromatin biology, enzyme activity, protein–DNA interactions, and epigenetic modifications.
How is rNuQ nucleosome concentration determined?
Following nucleosome assembly, we carefully quantify their concentration by measuring the OD of the nucleosome solution at 260nm, ensuring that the reading reflects fully assembled nucleosome particles rather than the individual components initially mixed during assembly. To further guarantee quality and integrity, we verify nucleosome formation with native PAGE, giving confidence that every batch contains correctly assembled, functional nucleosomes ready for precise studies in chromatin biology, epigenetics, and protein–DNA interactions
What advantages do recombinant nucleosomes offer compared to histone peptides or free histones?
Recombinant nucleosomes provide a complete and physiologically relevant chromatin substrate by combining histones and DNA into their native structural organization. Unlike histone peptides or free histones, which lack chromatin context, recombinant nucleosomes preserve critical histone–DNA interactions and nucleosomal architecture that regulate protein binding and enzymatic activity. This structural integrity enables more accurate, reproducible, and biologically meaningful results, making recombinant nucleosomes particularly well suited for studying chromatin-associated proteins, epigenetic modifications, and regulatory mechanisms under conditions that closely mimic native chromatin.
How should I store my rNuQ recombinant nucelosome?
Recombinant nucleosomes should be stored at +4°C (2-8°C) and must not be frozen, as freezing can affect their structural integrity. When properly stored, they are stable for up to 6 months after receipt.
What should I do if my rNuQ product doesn’t work as expected?
If your rNuQ product does not perform as expected, we recommend first reviewing the technical data sheet and protocol to ensure that storage conditions, handling procedures, and experimental parameters match the recommended guidelines. Including appropriate positive and negative controls can help determine whether the issue is assay-related or product-related. We also suggest verifying buffer conditions, protein concentrations, and experimental setup, as variations in these factors may affect performance. If the issue persists, please fill out the General Inquiry form with details of your experimental conditions, and we will be happy to assist you in troubleshooting and optimizing your experiment.
Do rNuQ products have expiration dates?
Yes. To ensure optimal performance and product integrity, rNuQ products are guaranteed stable for up to 6 months after delivery when stored as recommended.
How do I cite rNuQ products in my published research?
Proper citation ensures reproducibility and acknowledges the source of the reagents. When publishing your work, please cite rNuQ products by their full name, the catalog number and include the company name (Belgian Volition) with its location (Belgium) in the Materials and Methods section.
Can I request a custom nucleosome?
Yes! Please contact our team via the General Inquiry form to discuss your custom nucleosome requirements.
When performing a Western blot on rNuQ recombinant nucleosomes, I sometimes see two bands around 25-30 kDa. What does this mean?
Core histones have an expected molecular weight of 10–15 kDa, but in recombinant nucleosome preparations, it is common to observe two bands around 25–30 kDa on SDS-PAGE. This is due to the highly interactive nature of histones, which can form stable dimers or small complexes that are not fully disrupted during electrophoresis. This phenomenon is a technical feature of the Western blot and does not indicate multiple nucleosome species or impurities; native gel analysis confirms that a single nucleosome species is present.